PINK1 and Parkinson disease: To investigate the functional impact of PD mutations at the interface, we generated stable cell lines in which we re-introduced full-length PINK1-3FLAG WT; kinase-inactive mutant PINK1 (KI); NTE domain PINK1 mutants namely C125G, Q126P; CTE mutants PINK1 534_535InsQ and L539F, and kinase domain mutants namely A168P, E240 K, G309D and G409 V, into Flp-In T-REx HeLa PINK1-knockout cells (generated by exon 2-targeted CRISPR-Cas9 (electronic supplementary material, figure S8A,B)).