RNF43 and cancer: Interestingly, the immunoblotting results showed significantly higher active β‐catenin levels in CRC organoids with APC two‐hit mutations (C15, C46, and C48) than in those with RNF43 frameshift mutations (C14, C24, and C45) (Figure 2D and supplementary material, Table S2), which is consistent with the nuclear β‐catenin accumulation noted in APC‐mutant cancer cells (Figure 1A).