Based on this preliminary validation, more accurate potential miRNA-mRNA regulatory axes contributing to ALI were established, including the mmu-mir-7b-5p-Gria1, mmu-mir-486a-5p-Shc4 and mmu-mir-486b-5p-Shc4 regulatory pathways, which could first be further studied in clinical and basic experiments. The gene discussed is GRIA1; the disease is acute respiratory distress syndrome.