In this study, we confirmed the aberrant overexpression and prognostic role of SLCO4A1-AS1 in CRC using multiple cohorts and revealed that decreased DNA methylation levels in the promoter of SLCO4A1-AS1 account for, at least partly, the aberrant overexpression of SLCO4A1-AS1 in CRC. This evidence concerns the gene SLCO4A1 and colorectal carcinoma.