To verify the effect of mutant SF3B1 on DLG1 in vitro, we infected primary cultured pituitary tumor cells with adenovirus carrying the SF3B1-R625H mutation, and the results showed that the cryptic DLG1 transcript was observed in the Ad-SF3B1-R625H group (Fig. 3C). Here, SF3B1 is linked to pituitary tumor.