Finally, we evaluated the killing effect of DL35D-DM1 on other human cell lines as controls, including a human T cell line (MT-2), a human rhabdomyosarcoma cell line (RD), a human hepatocyte cell line (Huh-7), and a human glioblastoma cell line (U87 CD4+CCR5+), which are derived from primary cells in different human tissues. Here, CD4 is linked to rhabdomyosarcoma.