Herein, we exploited the 3WJ-pRNA structure to construct Cy3-labeled negatively charged and US-responsive A10-3.2/siCAT-1/3WJ-NDs bearing an prostate specific membrane antigen (PSMA)-targeting RNA aptamer and siCAT-1 to silence CAT-1 expression in prostate cancer cells. The gene discussed is SLC7A1; the disease is prostate cancer.