Using IHC staining of tumor sections with Ki67, there was a higher proportion of cycling tumor cells after IRFA compared with cells before IRFA, and the cycling cells after IRFA were predominantly located in the transition zone (TZ) between ablated zones and the residual tumors (Figure S1B, Supporting Information), which was consistent with previous studies, showing the aggressive states of tumor cells.[9]. The gene discussed is MKI67; the disease is neoplasm.