Differential gene expression analysis of our RNA-seq data showed that 53BP1−/− MEFs enriched cellular processes associated with response to Staphylococcus aureus infection and osteoclast differentiation, and downregulated cellular pathways such as DNA replication, cell cycle, and pyrimidine metabolism (Supplementary Figure S4A and B). Here, TP53BP1 is linked to staphylococcus aureus infection.