In this study, CHD7 was predicted as the target of miR-21-5p, mutations of this gene cause gonadal endocrine abnormalities, which manifests biochemically as hypogonadotropic hypogonadism (low sex steroid hormone and gonadotropin levels) (Kim et al., 2008; Neocleous et al., 2020; Xu et al., 2018). This evidence concerns the gene CHD7 and hypogonadotropic hypogonadism.