In order to study the role of Smad7 in myocardial fibrosis, we transfected pcDNA3.1 or pcDNA3.1-Smad7 into LPS-stimulated H9C2 cells, the expression level of Smad7 in the pcDNA3.1-Smad7 group was higher than that in the pcDNA3.1 group (Fig. 3A). This evidence concerns the gene SMAD7 and Myocardial fibrosis.