Given that triglycerides are mainly derived from the diet, and brain-derived sphingolipids could get enriched in the serum due to their higher levels in the brain than the serum, we further quantified sphingolipids from the same serum samples through a targeted lipidomic analysis, which identified 3 species of ceramides, namely Cer (d18:1_24:1), Hex1Cer (d16:0_22:6), Hex2Cer (d28:1) increased in the MCI group compared to NC group of T2DM patients. This evidence concerns the gene CBLN1 and type 2 diabetes mellitus.