The level of miR-3194-5p was significantly reduced in A549-T24 cells and E2-A549 cells (Fig. 8B(a) and C(a)); in addition, Sp1 positively regulated all the miRNAs, especially miR-3194-5p in E2-A549 and A549-T24 lung cancer cells (Fig. 8B(b) and C(b)). The gene discussed is SP1; the disease is lung carcinoma.