Considering growing evidence on aberrant use of multiple promoters in malignant cell and the importance of SMAD4 for malignant transformation, the aim of this study was to investigate the relative abundance of the transcript SMAD4–201 in colorectal cell lines and tissues, and also in development and under stress, in order to establish if its fluctuations may be associated with CRC. The gene discussed is SMAD4; the disease is colorectal carcinoma.