Of the analyzed malignant cell lines, DLD-1 belongs to the consensus molecular subtype of colorectal cancer CMS1, while HCT116, Caco2, SW480 and SW620 belong to the CMS4 group, and no significant difference was observed in SMAD4–201 abundance between these two CMS groups [28, 29]. The gene discussed is SMAD4; the disease is colorectal cancer.