To further explore whether miR-129-5p affected the cardiac function in CHF rats through Smurf1, the rats were transfected and allocated into the following groups after modeling: agomiR-NC + oe-NC group, agomiR-NC + oe-Smurf1 group, miR-129-5p agomiR + oe-NC group, and miR-129-5p agomiR + oe-Smurf1 group. Here, SMURF1 is linked to congestive heart failure.