We construct a Pig-a CKO mice model mediated by Vav-iCre Through the detection of various indicators in mice, it was proved that this model not only had stable GPI deficient but also had the disease phenotype of mild intravascular hemolysis, which may be an ideal animal model for PNH and can be used in the related studies on the pathogenesis and treatment of PNH. The gene discussed is VAV1; the disease is paroxysmal nocturnal hemoglobinuria.