In CKO mice, anemia, mild increase of LDH, TBIL and IBIL, increased level of free hemoglobin and complement C5b-9, and increased number of hemosiderin granules in spleen suggested the presence of intravascular hemolysis, indicating the successful establishment of a Pig-a conditional knock-out mice model mediated by Vav-iCre which had stable GPI-deficient and mild hemolysis. This evidence concerns the gene VAV1 and anemia.