To investigate whether PCMT1 plays roles in ovarian cancer through LAMB3, we knocked down LAMB3 in control and PCMT1-OE cells with specific siRNA targeting LAMB3. As shown in Fig. 4E and F, silencing of LAMB3 reduced the cell spheroid formation and migration of SKOV3 cells with PCMT1 overexpression, suggesting that LAMB3 acts downstream of PCMT1. This evidence concerns the gene PCMT1 and ovarian carcinoma.