TGFB1 and cholangiocarcinoma: However, anti-TGFβ1 neutralizing antibody or LY2157299 treatment suppressed the above EMT changes, TGFβ1 treatment alone acted as a positive control, suggesting that M2 macrophages-derived TGFβ1 induced CCA cell EMT (Fig. 4E-F and Supplementary Fig. 4D-E).