In order for the panel to be used in MPN diagnostics, optimisation of the sequencing coverage for the MPL region is critical for the detection of driver mutations in MPL. In addition, AMPL89337 (DNMT3A exon 17, chr2:25464411–25464625), AMPL1156 (TP53 exon 4/exon 5 chr17:7578360–7578579) and AMPL90417 (ASXL1 exon 13, chr20:31022935–31023187) were found to have average depths-of-coverage of < 1000x (Additional file 8: Fig. S2, Additional file 10). The gene discussed is DNMT3A; the disease is myeloproliferative neoplasm.