In addition, the expression of NEDD4 and KLF8 was elevated in bladder cancer cell lines (EJ-m3, T24, J82, BIU-87, and SW780) compared to that of the normal urothelial cell line SVHUC-1, with the highest expression in T24 cells, which were therefore selected for subsequent experiments (Fig. 1d). Here, KLF8 is linked to urinary bladder cancer.