For the purpose of evaluating the concrete function of ENAH on the malignant phenotypes of HCC cells, ENAH was knocked down in SNU-387 cells by transfection of shRNA-ENAH-1 and shRNA-ENAH-2 and the interference efficiency was tested by RT-qPCR and Western blot analysis (Figure 2a-b). Here, ENAH is linked to hepatocellular carcinoma.