At the point of confluence (pre‐differentiation, D0), markers of proliferation (Ki‐67, Myf‐5) were noted to be highly expressed in both cell populations with no differences seen in Ki‐67 mRNA expression, but significantly higher expression of Myf‐5 seen in cells derived from CKD patients (P = 0.0004). Here, MYF5 is linked to chronic kidney disease.