,102,106,177, 178, 179 These cell lines provide relatively stable models to study the effects of the presence of the mutIDH1/2 enzymes, but it is possible that the process of producing the model itself may have unknown metabolic consequences and that these models do not account for some genetic and, subsequently, metabolic differences between WT IDH and mutIDH1/2 gliomas.180, 181, 182 A limited number of glioma cell lines that endogenously express mutIDH have been successfully cultured from grade II astrocytomas,183 grade III gliomas, and what were formerly known as secondary GBMs.174. Here, IDH2 is linked to central nervous system cancer.