To determine the specific role of TMEM60 in glioma carcinogenesis, we conducted knockdown and overexpression of TMEM60 in glioma cell lines and confirmed that TMEM60 overexpression led to enhanced cell viability, proliferation, migration, and invasion, as well as decreased cell apoptosis, which is consistent with the abnormal low level of TMEM60 in glioma tumor sections. This evidence concerns the gene TMEM60 and glioma.