As shown in Figures 4(a) and 4(b), the results of western blotting showed that the protein expression levels of Gli and Bcl-2 in melanoma A375 cells are not low, but after treatment with cyclopamine (at a concentration of 40 μM), the protein expression levels of Gli and Bcl-2 present a downward trend (P < 0.05). This evidence concerns the gene GLI1 and melanoma.