Based on this, the main experimental method is to use cyclopamine, a specific blocker of the downstream molecule SMO of the Hedgehog signaling pathway, to treat cells, inhibit the activity of the signaling pathway, and observe its effects on the proliferation and apoptosis of melanoma A375 cells, to further clarify whether the Hedgehog signaling pathway regulates the proliferation and apoptosis of melanoma A375 cells. This evidence concerns the gene SMO and melanoma.