RSPO2 and infection: When pAAV–CAG–Rspo2 was injected into one depot of ingWAT in mice, while the other depot received pAAV–CAG–GFP as a control (Fig. 5g), RSPO2 protein levels were twofold higher (Fig. 5h,i), whereas mRNA levels were around 100-fold higher than pAAV–CAG–GFP-injected depots (Fig. 5j) 6 weeks after infection.