Oct3/4 expression was successfully manipulated by either Oct3/4 overexpression or knockdown lentivirus, and we found that Oct3/4 expression after HG treatment (HG(4d)+LG(4d)/↑Oct3/4) completely reversed, while Oct3/4 knockdown after LG treatment (LG(4d)+LG(4d)/shOct3/4) mimicked, hyperglycemia treatment (HG(4d)+LG(4d)/CTL)-mediated RORA suppression compared to the control (LG(4d)+LG(4d)/CTL) group (see Fig. 2g–i, Supplementary Fig. 1b). Here, POU5F1 is linked to Hyperglycemia.