To compare the abilities of educating HSCs into CAFs among the four CRC cell-derived exosomes, the expression of α-SMA and FAP in treated HSCs were evaluated by western blot, which showed most highly in LX-2 cells incubated with HCT116-derived exosomes (ExoHCT116) (Fig. 1B). This evidence concerns the gene ACTA1 and colorectal carcinoma.