Subsequently, to confirm that HSPC111 in exosomes derived from CRC cells affects acetyl-CoA level in LX-2 cells by interacting ACLY, we employed ACLY silencing (shACLY) LX-2 cells (Supplementary Fig. 2C) and found that incubation with ExoHCT116 significantly decreased acetyl-CoA level and increased citrate level in shACLY LX-2 cells (Fig. 4F). This evidence concerns the gene NOP16 and colorectal carcinoma.