Prnp+/+ mice spent the same amount of time in the two regions, while PrnpZH3/ZH3 animals remained significantly more time in the periphery close to the walls, suggesting an apprehension of the center of the field that reflects an anxiety-like behavior (Prnp+/+: Center = 303.3 ± 14.5 vs. Periphery = 345.7 ± 18,0; and PrnpZH3/ZH3: Center = 288.2 ± 15.1 vs. Periphery = 394.3 ± 20.3; mean ± S.E.M., p = 0.071 and ***p < 0.001 respectively; Mann-Whitney U non-parametric test) (Fig. 1d). Here, PRNP is linked to Anxiety.