Effect of miR-27a-3p downregulation on integrity and permeability of the endothelial barrier was examined using the TEER and the dextran permeability assays respectively, while gene and protein expression of essential TJ (claudin-5, occludin, ZO-1) and AJ (VE-cadherin) proteins highly enriched at the brain endothelium and known to be deregulated in multiple neurological disorders was examined using real time PCR and western-blot. Here, CLDN5 is linked to nervous system disorder.