These findings suggest that loss of endothelial miR-27a-3p may be a critical event causing upregulation of GSK3ß, downregulation of Wnt/ß-catenin and dysfunction of the inter-endothelial junctions, claudin-5 and occludin, at the brain endothelial barrier in neurological disorders. This evidence concerns the gene CLDN5 and nervous system disorder.