Interestingly, CRISPR/Cas9-mediated knockout of CTL4 resulted in the melanization of P. falciparum even at the low intensity infection, in contrast to studies based on RNAi-mediated CTL4 silencing, in which P. falciparum melanization was shown to be infection intensity-dependent and hence only observed with a high intensity infection [20]. Here, SLC44A4 is linked to infection.