Of note, we also tested the effect of DCA on endogenous APP processing in two additional unrelated cell lines (SW480 colon cancer and HEK293 cells) and demonstrated similar enhancements in the non-amyloidogenic processing of endogenous APP (although neither of these cell lines generate quantifiable endogenous levels of amyloidogenic processing proteolytic fragments) (S1 Fig). This evidence concerns the gene APP and malignant colon neoplasm.