To investigate whether overexpression of the catalytically dead ATP11B could phenocopy ATP11Blo or ATP11B-MT state in PS displacement and tumor metastasis, we generated an E186K mutation in mouse ATP11b, which is equivalent to human E180K in the catalytic domain of ATP11B (Supplemental Figure 2E), and compared the effect of sgATP11b and ATP11b-E186K in 545 cells. This evidence concerns the gene ATP11B and neoplasm.