Furthermore, pharmacological inhibition of PIKFYVE activity by inhibitor YM201636 also induced aberrant vacuolation in human lens epithelial cells HLEB3 (Figure 3E and F), suggesting that the function of PIKFYVE in the regulation of cataract formation is highly conserved across species and our zebrafish cataract model would potentially be an excellent system for further preclinical study. This evidence concerns the gene PIKFYVE and cataract.