TWIST1 and neoplasm: To validate the tumor-stroma signature at the protein level and further characterize TWIST1-mediated alterations in cell−cell communication, we analyzed the secretome of SK-N-Be2c-Control and -sgTWIST1 cells in vitro by HPLC/Tandem MS using their conditioned media (CM) containing both secreted proteins and extracellular vesicles released by tumor cells.