Even though the addition of recombinant TIMP-2 induced resistance to 5-Fu in DLD-1 5-FuS and HCT116 5-FuS cells containing ERK/MAPK, TIMP-2 did not induce 5-Fu resistance in CRC cells with downregulated ERK/MAPK signaling pathway (Figure 7C and 7D). Here, MAPK1 is linked to colorectal carcinoma.