Reducing gene expression from a high to a moderate level has smaller effects than increasing gene expression from a low to a high level.38 Accordingly, KD of the highly expressed CCT3 caused a small increase of LINC00326 transcript abundance, while CRISPRa-mediated OE substantially stimulated LINC00326 gene expression and could explain why more genes were affected by LINC00326-OE than CCT3-KD in liver cancer cells. The gene discussed is CCT3; the disease is liver cancer.