EGR1 is in itself a TF, and accumulating evidence substantiates its tumour suppressing role in HCC.43 Loss of EGR1 and tumour development are connected through oncogenic RAS-PI3K signalling44, which is also a top GO term that we identified after LINC00326-OE (online supplemental figure S7). This evidence concerns the gene EGR1 and hepatocellular carcinoma.