The tumor immune microenvironment of ESCC has been reported to be in an immunosuppressive state dominated by exhausted T and natural killer (NK) cells.32 In the present study, there were few tumor-infiltrating immune cells before treatment; however, a significant increase in tumor-infiltrating CD4+, CD8+, and CD56+ lymphocytes was observed after therapy. Here, CD8A is linked to esophageal squamous cell carcinoma.