To determine the impact of EV infection on the development of ALS, the neonates (2–3 days) of transgenic mice carrying human mutant SOD1 (SOD1G85R) and non-transgenic C57BL/6J (background strain used as experimental control) mice were intracerebroventricularly inoculated with a sublethal dose of CVB3 (500 pfu) or an equal volume of DMEM (mock infection) for 10 days, 20 weeks or 60 weeks (Fig. 1A). Here, SOD1 is linked to infection.