The amount of total AMPKα1/2 and phosphorylated AMPKα1/2 on Thr172 (P-AMPKα1/2) in HDFs at different stages (Y: young cells with PD 12, M: middle cells with PD 48, and S: senescent cells with PD 86) were assessed via western blot analysis. The gene discussed is PRKAA1; the disease is Parkinson disease.