Following this pathway in DLBCL cells by Western blot analysis after SIRT3 knockdown confirmed that reduction of ATF4 was accompanied by induction of autophagy as shown by LC3II/LC3I ratios as well as reduction of histone acetylation, which reflects impaired production of AcCoA from the TCA cycle (ref. 14; Supplementary Fig. S5A). The gene discussed is ATF4; the disease is diffuse large B-cell lymphoma.