In infection by pseudoviruses with alphavirus envelopes that are initially expressed as polyproteins with multiple transmembrane domains (Fig. 1I), Chikungunya virus (CHIKV) envelope was found to be especially highly susceptible to MARCH8 (Fig. 1J), whereas the Ross River virus (RRV) envelope, was also dose-dependently inhibited (Fig. 1K). This evidence concerns the gene MARCHF8 and infection.