As shown in Table 2, compared with the control group, 9 markers were overexpressed in NSCLC patients, including soluble TIM‐3, CD137, CD27, LAG‐3, IDO, PD‐L2, CD152, CD80, and PD‐1 (0.92, 0.27, 74.15, 2.68, 6.11, 760.00, 1.65, 6.32, and 7.29 pg/ml, respectively). The gene discussed is CD80; the disease is non-small cell lung carcinoma.