Besides, it has been proved that sustained peripheral expression using recombinant adeno-associated virus (rAAV) vectors of transgene adiponectin offsets the development of diet-induced obesity [228]. In the latter study conducted at the University of Florida, where one of the authors of the current review took part as an integral part of the collaborative research team, the long-term (up to 280 days) expression of AAV-Acrp30 vectors encoding mouse Acrp30 cDNA was tested after intramuscular and intraportal injections in female Sprague-Dawley rats. This evidence concerns the gene ADIPOQ and obesity due to melanocortin 4 receptor deficiency.