To better understand the underlying mechanisms of improved poststroke cognitive function recovery in the Nhe1 cKO mice, we performed microglial transcriptomic analysis by bulk RNAseq of P2RY12+ microglia isolated by magnetically-activated cell sorting (MACS) from non-lesion (contralateral, CL) and ischemic (ipsilateral, IL) hemispheres of WT and cKO brains at 3-day post-stroke (Fig. 2a). This evidence concerns the gene SLC9A1 and stroke disorder.