ATRX SNV mutation frequency was associated with decreased mRNA abundance in all three subtypes, and we also found a significant age-ATRX interaction in astrocytoma, where ATRX- was associated with a greater decreased in mRNA abundance in tumours of older individuals than younger (interaction p = 0.016, Supplementary Fig. 5). Here, ATRX is linked to astrocytoma (excluding glioblastoma).