We identify a splice-switching ASO strategy that induces exon 23 skipping and show that ASO-mediated exon 23 skipping in CFTR-W1282X RNA, in both an immortalized human CFTR-W1282X bronchial epithelial cell line and primary epithelial cells isolated from a CF patient homozygous for CFTR-W1282X, stabilizes the CFTR mRNA and recovers CFTR activity. Here, CFTR is linked to cystic fibrosis.