To identify the binding site(s) for β-catenin/TCF/LEF in the ZNF277 promoter in HT29 human colon cancer cells with robust β-catenin and ZNF277 expression, we performed ChIP assays using quantitative PCR (qPCR) primers for each potential site (Supplemental Table 2) with ChIP-grade anti–β-catenin antibodies. Here, ZNF277 is linked to colonic neoplasm.