PCSK9 and atherosclerosis: We used a novel model of atherosclerosis that utilises a single adeno-associated virus (AAV) i.v. injection of a gain-of-function mutation (D377Y) to proprotein convertase subtilisin/kexin type 9 (rAAV8-mPCSK9-D377Y), combined with a high-fat Western diet to induce atherosclerosis in most adult mouse strains (Bjørklund et al., 2014; Roche-Molina et al., 2015).