LGR5 and neoplasm: To test if the methylation profile of TEs follows the trend observed genome-wide, the genomic coordinates that correspond to the 1 kb transcription start site (TSS) region (namely, +/− 500 bps flanking the most 5′ ends) of Alu and full-length LINE-1 in hg38 repeat regions were extracted and the relevant methylation levels were compared between OS tumor samples and normal controls.